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pegfp n1 mammalian expression vector  (TaKaRa)


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    TaKaRa pegfp n1 mammalian expression vector
    Pegfp N1 Mammalian Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 2461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+mammalian+expression+vector/Nhe+I/bio_rxiv__2025__02__10__637577-293-27-32
    Average 96 stars, based on 2461 article reviews
    pegfp n1 mammalian expression vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cloning:

    Article Title: Compositions and methods for recombinant synthesis of high molecular weight hyaluronic acid
    Article Snippet: .. To demonstrate cloning and expression of functional naked mole rat HAS2 in an illustrative recombinant expression system as generally described above, the HAS2 cDNA (SEQ ID NO:2) was cloned in pEGFP-N1 mammalian expression vector (CLONTECH) under CMV promoter control; the cDNA replaced EGFP. ..

    Article Title: TMEM145 is a key component in stereociliary link structures of outer hair cells
    Article Snippet: Human TMEM145 (NM_173633.3) and mouse Tmem145 (NM_183311.3) coding regions were purchased from OriGene (Rockville, MD, USA). .. Each coding region was amplified using PCR to introduce a C-terminal FLAG tag (DYKDDDK) immediately upstream of the stop codon, and the fragments were subcloned into the pEGFP N1 mammalian expression vector (Clontech, USA) using the NheI and NotI restriction sites via In Fusion Cloning (Takara Bio, Japan). ..

    Expressing:

    Article Title: Compositions and methods for recombinant synthesis of high molecular weight hyaluronic acid
    Article Snippet: .. To demonstrate cloning and expression of functional naked mole rat HAS2 in an illustrative recombinant expression system as generally described above, the HAS2 cDNA (SEQ ID NO:2) was cloned in pEGFP-N1 mammalian expression vector (CLONTECH) under CMV promoter control; the cDNA replaced EGFP. ..

    Article Title: TMEM145 is a key component in stereociliary link structures of outer hair cells
    Article Snippet: Human TMEM145 (NM_173633.3) and mouse Tmem145 (NM_183311.3) coding regions were purchased from OriGene (Rockville, MD, USA). .. Each coding region was amplified using PCR to introduce a C-terminal FLAG tag (DYKDDDK) immediately upstream of the stop codon, and the fragments were subcloned into the pEGFP N1 mammalian expression vector (Clontech, USA) using the NheI and NotI restriction sites via In Fusion Cloning (Takara Bio, Japan). ..

    Article Title: Enhanced generation of Alzheimer's amyloid-? following chronic exposure to phorbol ester correlates with differential effects on alpha and epsilon isozymes of protein kinase C
    Article Snippet: .. The amplified fragment was then subcloned into the pEGFP-N1 mammalian expression vector (Clontech Laboratories Inc, Mountain View, CA, USA) and fully sequenced prior to use ( da Cruz e Silva et al . 2004 ). ..

    Article Title: Structural Characterization of a Human Cytosolic NMN/NaMN Adenylyltransferase and Implication in Human NAD Biosynthesis
    Article Snippet: Nickel-nitrilotriacetic acid-agarose was from Qiagen (Valencia, CA). .. The pEGFP-N1 mammalian expression vector was from Clontech (Palo Alto, CA). .. The MitoTraker Red mitochondria-specific fluorescent dye was purchased from Molecular Probes (Eugene, OR).

    Article Title: Tyrosine 687 phosphorylated Alzheimer's amyloid precursor protein is retained intracellularly and exhibits a decreased turnover rate.
    Article Snippet: Tyrosine 687 (Y687) of the Alzheimer’s amyloid precursor protein (APP) was shown to be phosphorylated in the brains of Alzheimer’s disease patients.. This residue lies within a typical endocytosis consensus motif commonly found in molecules with receptor functions, strongly suggesting a potential role for APP in signal transduction.. Consequently, the work here described addressed how phosphorylation of Y687 may be affecting APP in terms of its proteolytic cleavage and subcellular distribution.

    Article Title: FcRH1: an activation coreceptor on human B cells.
    Article Snippet: B-cell activation and differentiation is regulated through the coordinated function of a dynamic array of cell surface receptors.. At different stages in their differentiation, human B cells may express one or more members of a large family of immunoglobulin Fc receptor homologs (FcRH) with regulatory potential.. Among these newly identified transmembrane molecules, FcRH1 is unique in having 2 immunoreceptor tyrosine-based activation motif (ITAM)–like motifs in its intracellular domain.

    Article Title: Equine Viperin Restricts Equine Infectious Anemia Virus Replication by Inhibiting the Production and/or Release of Viral Gag, Env, and Receptor via Distortion of the Endoplasmic Reticulum
    Article Snippet: .. Tat was amplified from EIAV-CMV-3-8 and cloned into the pEGFP-N1 mammalian expression vector (Clontech, USA) with a hemagglutinin (HA) tag. ..

    Article Title: Molecular Insights into Mammalian End-binding Protein Heterodimerization
    Article Snippet: .. Mammalian Expression Constructs and Plasmid Transfection For mammalian expression of EB full-length FRET constructs, the cloning cassettes of the bacterial expression vectors (see above) were inserted into the backbone of the pEGFP-N1 mammalian expression vector (Clontech). ..

    Functional Assay:

    Article Title: Compositions and methods for recombinant synthesis of high molecular weight hyaluronic acid
    Article Snippet: .. To demonstrate cloning and expression of functional naked mole rat HAS2 in an illustrative recombinant expression system as generally described above, the HAS2 cDNA (SEQ ID NO:2) was cloned in pEGFP-N1 mammalian expression vector (CLONTECH) under CMV promoter control; the cDNA replaced EGFP. ..

    Recombinant:

    Article Title: Compositions and methods for recombinant synthesis of high molecular weight hyaluronic acid
    Article Snippet: .. To demonstrate cloning and expression of functional naked mole rat HAS2 in an illustrative recombinant expression system as generally described above, the HAS2 cDNA (SEQ ID NO:2) was cloned in pEGFP-N1 mammalian expression vector (CLONTECH) under CMV promoter control; the cDNA replaced EGFP. ..

    Clone Assay:

    Article Title: Compositions and methods for recombinant synthesis of high molecular weight hyaluronic acid
    Article Snippet: .. To demonstrate cloning and expression of functional naked mole rat HAS2 in an illustrative recombinant expression system as generally described above, the HAS2 cDNA (SEQ ID NO:2) was cloned in pEGFP-N1 mammalian expression vector (CLONTECH) under CMV promoter control; the cDNA replaced EGFP. ..

    Article Title: Equine Viperin Restricts Equine Infectious Anemia Virus Replication by Inhibiting the Production and/or Release of Viral Gag, Env, and Receptor via Distortion of the Endoplasmic Reticulum
    Article Snippet: .. Tat was amplified from EIAV-CMV-3-8 and cloned into the pEGFP-N1 mammalian expression vector (Clontech, USA) with a hemagglutinin (HA) tag. ..

    Article Title: Molecular Insights into Mammalian End-binding Protein Heterodimerization
    Article Snippet: .. Mammalian Expression Constructs and Plasmid Transfection For mammalian expression of EB full-length FRET constructs, the cloning cassettes of the bacterial expression vectors (see above) were inserted into the backbone of the pEGFP-N1 mammalian expression vector (Clontech). ..

    Plasmid Preparation:

    Article Title: Compositions and methods for recombinant synthesis of high molecular weight hyaluronic acid
    Article Snippet: .. To demonstrate cloning and expression of functional naked mole rat HAS2 in an illustrative recombinant expression system as generally described above, the HAS2 cDNA (SEQ ID NO:2) was cloned in pEGFP-N1 mammalian expression vector (CLONTECH) under CMV promoter control; the cDNA replaced EGFP. ..

    Article Title: TMEM145 is a key component in stereociliary link structures of outer hair cells
    Article Snippet: Human TMEM145 (NM_173633.3) and mouse Tmem145 (NM_183311.3) coding regions were purchased from OriGene (Rockville, MD, USA). .. Each coding region was amplified using PCR to introduce a C-terminal FLAG tag (DYKDDDK) immediately upstream of the stop codon, and the fragments were subcloned into the pEGFP N1 mammalian expression vector (Clontech, USA) using the NheI and NotI restriction sites via In Fusion Cloning (Takara Bio, Japan). ..

    Article Title: Enhanced generation of Alzheimer's amyloid-? following chronic exposure to phorbol ester correlates with differential effects on alpha and epsilon isozymes of protein kinase C
    Article Snippet: .. The amplified fragment was then subcloned into the pEGFP-N1 mammalian expression vector (Clontech Laboratories Inc, Mountain View, CA, USA) and fully sequenced prior to use ( da Cruz e Silva et al . 2004 ). ..

    Article Title: Molecular Insights into Mammalian End-binding Protein Heterodimerization
    Article Snippet: .. Mammalian Expression Constructs and Plasmid Transfection For mammalian expression of EB full-length FRET constructs, the cloning cassettes of the bacterial expression vectors (see above) were inserted into the backbone of the pEGFP-N1 mammalian expression vector (Clontech). ..

    Control:

    Article Title: Compositions and methods for recombinant synthesis of high molecular weight hyaluronic acid
    Article Snippet: .. To demonstrate cloning and expression of functional naked mole rat HAS2 in an illustrative recombinant expression system as generally described above, the HAS2 cDNA (SEQ ID NO:2) was cloned in pEGFP-N1 mammalian expression vector (CLONTECH) under CMV promoter control; the cDNA replaced EGFP. ..

    Amplification:

    Article Title: TMEM145 is a key component in stereociliary link structures of outer hair cells
    Article Snippet: Human TMEM145 (NM_173633.3) and mouse Tmem145 (NM_183311.3) coding regions were purchased from OriGene (Rockville, MD, USA). .. Each coding region was amplified using PCR to introduce a C-terminal FLAG tag (DYKDDDK) immediately upstream of the stop codon, and the fragments were subcloned into the pEGFP N1 mammalian expression vector (Clontech, USA) using the NheI and NotI restriction sites via In Fusion Cloning (Takara Bio, Japan). ..

    Article Title: Enhanced generation of Alzheimer's amyloid-? following chronic exposure to phorbol ester correlates with differential effects on alpha and epsilon isozymes of protein kinase C
    Article Snippet: .. The amplified fragment was then subcloned into the pEGFP-N1 mammalian expression vector (Clontech Laboratories Inc, Mountain View, CA, USA) and fully sequenced prior to use ( da Cruz e Silva et al . 2004 ). ..

    Article Title: Equine Viperin Restricts Equine Infectious Anemia Virus Replication by Inhibiting the Production and/or Release of Viral Gag, Env, and Receptor via Distortion of the Endoplasmic Reticulum
    Article Snippet: .. Tat was amplified from EIAV-CMV-3-8 and cloned into the pEGFP-N1 mammalian expression vector (Clontech, USA) with a hemagglutinin (HA) tag. ..

    Polymerase Chain Reaction:

    Article Title: TMEM145 is a key component in stereociliary link structures of outer hair cells
    Article Snippet: Human TMEM145 (NM_173633.3) and mouse Tmem145 (NM_183311.3) coding regions were purchased from OriGene (Rockville, MD, USA). .. Each coding region was amplified using PCR to introduce a C-terminal FLAG tag (DYKDDDK) immediately upstream of the stop codon, and the fragments were subcloned into the pEGFP N1 mammalian expression vector (Clontech, USA) using the NheI and NotI restriction sites via In Fusion Cloning (Takara Bio, Japan). ..

    Introduce:

    Article Title: TMEM145 is a key component in stereociliary link structures of outer hair cells
    Article Snippet: Human TMEM145 (NM_173633.3) and mouse Tmem145 (NM_183311.3) coding regions were purchased from OriGene (Rockville, MD, USA). .. Each coding region was amplified using PCR to introduce a C-terminal FLAG tag (DYKDDDK) immediately upstream of the stop codon, and the fragments were subcloned into the pEGFP N1 mammalian expression vector (Clontech, USA) using the NheI and NotI restriction sites via In Fusion Cloning (Takara Bio, Japan). ..

    FLAG-tag:

    Article Title: TMEM145 is a key component in stereociliary link structures of outer hair cells
    Article Snippet: Human TMEM145 (NM_173633.3) and mouse Tmem145 (NM_183311.3) coding regions were purchased from OriGene (Rockville, MD, USA). .. Each coding region was amplified using PCR to introduce a C-terminal FLAG tag (DYKDDDK) immediately upstream of the stop codon, and the fragments were subcloned into the pEGFP N1 mammalian expression vector (Clontech, USA) using the NheI and NotI restriction sites via In Fusion Cloning (Takara Bio, Japan). ..

    Construct:

    Article Title: Molecular Insights into Mammalian End-binding Protein Heterodimerization
    Article Snippet: .. Mammalian Expression Constructs and Plasmid Transfection For mammalian expression of EB full-length FRET constructs, the cloning cassettes of the bacterial expression vectors (see above) were inserted into the backbone of the pEGFP-N1 mammalian expression vector (Clontech). ..

    Transfection:

    Article Title: Molecular Insights into Mammalian End-binding Protein Heterodimerization
    Article Snippet: .. Mammalian Expression Constructs and Plasmid Transfection For mammalian expression of EB full-length FRET constructs, the cloning cassettes of the bacterial expression vectors (see above) were inserted into the backbone of the pEGFP-N1 mammalian expression vector (Clontech). ..



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